Full list of all 46 Slay the Spire achievements. It takes around 60-80 hours to unlock all of the achievements in the base game on Windows.
The base game contains 26 achievements, and there are 8 DLC packs containing 20 achievements.
Defeat a boss in a single turn.
Have 50 or more Strength during combat.
Play 10 Shivs in a single turn.
Have 999 Block during combat.
Have 10 or more buffs during combat.
Have 99 or more Block during combat.
Defeated the Awakened One.
Play 25 cards in a single turn.
Win a combat without playing an Attack.
Have 3 or fewer cards in hand, draw, and discard pile combined.
Beat the game with the Silent.
Have 9 Energy during a single combat.
Apply 99 or more Poison on a single enemy.
Exhaust 20 cards in a single combat.
Defeated the Time Eater.
Defeat 3 enemies with Poison in a single combat.
Win a battle with 1 HP remaining.
Defeated the Guardian.
Beat the game with the Ironclad.
Defeated Donu & Deca.
Defeated the Collector.
Defeated the Bronze Automaton.
Defeat a boss without taking any damage.
Defeated the Slime Boss.
Defeated the Champ.
Defeated Hexaghost.
Obtain all other Achievements.
Beat the game in under 20 minutes.
Complete Ascension Level 10.
Beat the game with a single relic.
Finish Donu with a Feed.
Beat the game with a 5 card deck or smaller.
Unlock Ascension mode.
Have 25 or more Focus during combat.
Beat the game with the Defect.
Beat the game with a deck of only common cards.
Channel 9 Plasma in a single turn.
Win a Daily Climb.
Defeat the Transient before it fades away.
Complete Ascension Level 20.
Complete the Ending with the Watcher.
Beat the game with the Watcher.