Full list of all 46 Slay the Spire achievements. It takes around 60-80 hours to unlock all of the achievements in the base game on Windows.
The base game contains 26 achievements, and there are 8 DLC packs containing 20 achievements.
Play 10 Shivs in a single turn.
Defeat a boss in a single turn.
Win a combat without playing an Attack.
Have 999 Block during combat.
Play 25 cards in a single turn.
Have 50 or more Strength during combat.
Have 3 or fewer cards in hand, draw, and discard pile combined.
Defeated the Awakened One.
Defeated the Champ.
Have 99 or more Block during combat.
Beat the game with the Silent.
Defeated Donu & Deca.
Apply 99 or more Poison on a single enemy.
Beat the game with the Ironclad.
Defeated the Time Eater.
Have 10 or more buffs during combat.
Exhaust 20 cards in a single combat.
Have 9 Energy during a single combat.
Defeated the Bronze Automaton.
Defeat a boss without taking any damage.
Defeat 3 enemies with Poison in a single combat.
Win a battle with 1 HP remaining.
Defeated the Collector.
Defeated Hexaghost.
Defeated the Slime Boss.
Defeated the Guardian.
Obtain all other Achievements.
Beat the game with a 5 card deck or smaller.
Beat the game with a single relic.
Finish Donu with a Feed.
Beat the game in under 20 minutes.
Unlock Ascension mode.
Complete Ascension Level 10.
Have 25 or more Focus during combat.
Beat the game with the Defect.
Beat the game with a deck of only common cards.
Channel 9 Plasma in a single turn.
Win a Daily Climb.
Defeat the Transient before it fades away.
Complete Ascension Level 20.
Beat the game with the Watcher.
Complete the Ending with the Watcher.