Full list of all 46 Slay the Spire achievements. It takes around 60-80 hours to unlock all of the achievements in the base game on Windows.
The base game contains 26 achievements, and there are 8 DLC packs containing 20 achievements.
Have 10 or more buffs during combat.
Defeat a boss in a single turn.
Have 50 or more Strength during combat.
Win a combat without playing an Attack.
Apply 99 or more Poison on a single enemy.
Play 10 Shivs in a single turn.
Play 25 cards in a single turn.
Have 3 or fewer cards in hand, draw, and discard pile combined.
Defeated the Champ.
Defeated the Time Eater.
Defeated the Collector.
Beat the game with the Ironclad.
Defeated Donu & Deca.
Defeat a boss without taking any damage.
Have 999 Block during combat.
Have 99 or more Block during combat.
Defeated the Slime Boss.
Defeated Hexaghost.
Beat the game with the Silent.
Defeated the Awakened One.
Win a battle with 1 HP remaining.
Defeated the Bronze Automaton.
Defeat 3 enemies with Poison in a single combat.
Have 9 Energy during a single combat.
Exhaust 20 cards in a single combat.
Defeated the Guardian.
Obtain all other Achievements.
Beat the game in under 20 minutes.
Finish Donu with a Feed.
Complete Ascension Level 10.
Beat the game with a single relic.
Beat the game with a 5 card deck or smaller.
Unlock Ascension mode.
Have 25 or more Focus during combat.
Beat the game with a deck of only common cards.
Beat the game with the Defect.
Channel 9 Plasma in a single turn.
Win a Daily Climb.
Defeat the Transient before it fades away.
Complete Ascension Level 20.
Complete the Ending with the Watcher.
Beat the game with the Watcher.