Full list of all 46 Slay the Spire achievements. It takes around 60-80 hours to unlock all of the achievements in the base game on Windows.
The base game contains 26 achievements, and there are 8 DLC packs containing 20 achievements.
Have 50 or more Strength during combat.
Apply 99 or more Poison on a single enemy.
Play 10 Shivs in a single turn.
Win a combat without playing an Attack.
Have 999 Block during combat.
Have 3 or fewer cards in hand, draw, and discard pile combined.
Defeat a boss in a single turn.
Defeated the Time Eater.
Defeated the Collector.
Have 9 Energy during a single combat.
Defeated the Slime Boss.
Beat the game with the Silent.
Defeated Donu & Deca.
Defeat a boss without taking any damage.
Defeated the Bronze Automaton.
Defeat 3 enemies with Poison in a single combat.
Defeated Hexaghost.
Win a battle with 1 HP remaining.
Beat the game with the Ironclad.
Defeated the Awakened One.
Exhaust 20 cards in a single combat.
Play 25 cards in a single turn.
Have 99 or more Block during combat.
Have 10 or more buffs during combat.
Defeated the Champ.
Defeated the Guardian.
Obtain all other Achievements.
Complete Ascension Level 10.
Finish Donu with a Feed.
Beat the game in under 20 minutes.
Beat the game with a single relic.
Beat the game with a 5 card deck or smaller.
Unlock Ascension mode.
Have 25 or more Focus during combat.
Beat the game with a deck of only common cards.
Beat the game with the Defect.
Channel 9 Plasma in a single turn.
Win a Daily Climb.
Defeat the Transient before it fades away.
Complete Ascension Level 20.
Complete the Ending with the Watcher.
Beat the game with the Watcher.