Here is the full list of all 133 Empires of the Undergrowth achievements.
Win level "New Home".
Win level "New Home" on Medium difficulty.
Win level "New Home" on Hard difficulty.
Win level "New Home" on Insane difficulty.
Do not upgrade any soldiers and win the level "New Home" on Medium difficulty.
Win the level "New Home" on hard difficulty within 17 minutes.
Win the level "New Home" with the last 10 tunneled out creatures being woodlice on Insane difficulty.
Win level "New Home" with the challenge active.
Win the level "New Home" before 10 mole crickets have attacked with the challenge active.
Win level "New Home" on Insane difficulty with the challenge active.
Win the level "New Home" after 50 mole crickets have attacked on Insane difficulty with the challenge active.
Win level "Subjugation".
Win level "Subjugation" on Medium difficulty.
Win level "Subjugation" on Hard difficulty.
Win level "Subjugation" on Insane difficulty.
Win the level "Subjugation" within 16 minutes on Medium difficulty.
Kill 10 funnel web spiders in the first 6 minutes, then win the level "Subjugation" on Hard difficulty.
Lose no more than 1 egg to the slave makers in level "Subjugation" on Insane difficulty.
Win level "Subjugation" with the challenge active.
Get a cricket to kill a funnel web spider in level "Subjugation".
Win level "Subjugation" on insane difficulty with the challenge active.
Win the level "Subjugation" whilst losing at least 50 eggs to the slave makers on Insane difficulty with the challenge active.
Survive the first experiments.Win "Formicarium Challenge 1".
Survive the first experiments.Win "Formicarium Challenge 1" on Medium difficulty.
Survive the first experiments.Win "Formicarium Challenge 1" on Hard difficulty.
Survive the first experiments.Win "Formicarium Challenge 1" on Insane difficulty.
Win "Formicarium Challenge 1" without unlocking black ants.
Kill all the Ubers in the Hungry Spider level.
Beat "New Home" on extreme difficulty.
Beat "New Home" on extreme difficulty without letting any enemies within 3 tiles of the queen.
Beat "Subjugation" on extreme difficulty.
Beat "Subjugation" on extreme difficulty after losing over 80% of the brood to a single wave of slave makers.
Beat "The First Experiment" on extreme difficulty.
Beat the level "Rising Tide".
Beat "Rising Tide" on medium difficulty.
Beat "Rising Tide" on hard difficulty.
Beat "Rising Tide" on extreme difficulty.
Beat "Rising Tide" on impossible difficulty.
Beat "Rising Tide" on medium difficulty after returning the full unharvested sardine to the ocean.
Beat "Rising Tide" on hard difficulty without enemies entering your nest.
Beat "Rising Tide" on extreme difficulty after eliminating two tiger beetles before the first night.
Beat "Rising Tide" on impossible difficulty after eliminating the large sand bear wolf spider to the south of the nest.
Beat "Rising Tide" with the challenge active.
Beat "Rising Tide" without building additional workers with the challenge active.
Beat "Rising Tide" on extreme difficulty with the challenge active.
Beat "Rising Tide" on impossible difficulty after eliminating 50 beach tiger beetle larvae with the challenge active.
Beat the level "Queen of the Hill".
Beat "Queen of the Hill" on medium difficulty.
Beat "Queen of the Hill" on hard difficulty.
Beat "Queen of the Hill" on extreme difficulty.
Beat "Queen of the Hill" on impossible difficulty.
Beat "Queen of the Hill" on medium difficulty after fending off two ladybirds within 30 seconds of each other from separate aphid clusters.
Beat "Queen of the Hill" on hard difficulty after harvesting and bringing home 100 food from a fish.
Beat "Queen of the Hill" on extreme difficulty without building any melee wood ant brood tiles.
Beat "Queen of the Hill" on impossible difficulty without upgrading any ranged wood ant brood tiles.
Beat "Queen of the Hill" with the challenge active.
Beat "Queen of the Hill" with the challenge active after eliminating all the tiger beetle larvae.
Beat "Queen of the Hill" on extreme difficulty with the challenge active.
Beat "Queen of the Hill" on impossible difficulty after eliminating 3 large hermit crabs with the challenge active.
Beat the formicarium challenge "The Second Experiment".
Beat "The Second Experiment" on medium difficulty.
Beat "The Second Experiment" on hard difficulty.
Beat "The Second Experiment" on extreme difficulty.
Beat "The Second Experiment" on impossible difficulty.
Beat "The Second Experiment" without enemies entering your nest.
Beat the level "The Harvest".
Beat "The Harvest" on medium difficulty.
Beat "The Harvest" on hard difficulty.
Beat "The Harvest" on extreme difficulty.
Beat "The Harvest" on impossible difficulty.
Beat "The Harvest" on medium difficulty by eliminating the enemy queen.
Beat "The Harvest" on hard difficulty before the enemy colony harvests 5000 leaves.
Beat "The Harvest" on extreme difficulty without digging out any underground enemies.
Beat "The Harvest" on impossible difficulty by gathering leaves within 43 minutes.
Beat "The Harvest" with the challenge active.
Beat "The Harvest" with no casualties from phorid flies with the challenge active.
Beat "The Harvest" on extreme difficulty with the challenge active.
Beat "The Harvest" on impossible difficulty after clearing 4 plants of phorid flies with the challenge active.
Beat the level "Front Line".
Beat "Front Line" on medium difficulty.
Beat "Front Line" on hard difficulty.
Beat "Front Line" on extreme difficulty.
Beat "Front Line" on impossible difficulty.
Beat "Front Line" on medium difficulty before the second night.
Beat "Front Line" on hard difficulty without any army ant scouts reporting back to their colony.
Beat "Front Line" on extreme difficulty without building any major ant brood tiles.
Beat "Front Line" on impossible difficulty after defeating the huge whip spider before the third day.
Beat "Front Line" with the challenge active.
Beat "Front Line" with the challenge active without building any level 3 ants.
Beat the level "Front Line" on extreme difficulty with the challenge active.
Beat "Front Line" on impossible difficulty after harvesting all the available leaves with the challenge active.
Beat the formicarium challenge "Possibly the Final Experiment".
Beat "Possibly the Final Experiment" on medium difficulty.
Beat "Possibly the Final Experiment" on hard difficulty.
Beat "Possibly the Final Experiment" on extreme difficulty.
Beat "Possibly the Final Experiment" on impossible difficulty.
Beat "Possibly the Final Experiment" after placing only a single marker down and not moving ants between groups.
Beat the "Festive Spider" level.
Beat the "Festive Spider" level with at least half the gifts remaining under the tree.
Beat the "Festive Spider" level with all gifts remaining under the tree.
Beat the level "New Year's Feast".
Beat "New Year's Feast" on medium difficulty.
Beat "New Year's Feast" on hard difficulty.
Beat "New Year's Feast" on extreme difficulty.
Beat "New Year's Feast" on impossible difficulty.
Beat "New Year's Feast" on medium difficulty after rescuing the guests to the west of the nest without engaging the velvet worm.
Beat "New Year's Feast" on hard difficulty after cutting the long journey in half with a firecracker.
Beat "New Year's Feast" on extreme difficulty after eliminating all underground enemies.
Beat "New Year's Feast" on impossible difficulty, receive all guests and keep them alive until the end of the level.
Beat the level "Cold Blood".
Beat "Cold Blood" on medium difficulty.
Beat "Cold Blood" on hard difficulty.
Beat "Cold Blood" on extreme difficulty.
Beat "Cold Blood" on impossible difficulty.
Beat "Cold Blood" on medium difficulty with both enemy colonies still alive.
Beat "Cold Blood" on hard difficulty after tunnelling out all underground food by the second night.
Beat "Cold Blood" on extreme difficulty after defeating both of the enemy colonies
Beat "Cold Blood" on impossible difficulty after alerting both enemy colonies to your presence within the first 5 minutes.
Beat the level "A Bridge Too Far".
Beat "A Bridge Too Far" on medium difficulty.
Beat "A Bridge Too Far" on hard difficulty.
Beat "A Bridge Too Far" on extreme difficulty.
Beat "A Bridge Too Far" on impossible difficulty.
Beat "A Bridge Too Far" on medium difficulty after collecting all the aphids.
Beat "A Bridge Too Far" on hard difficulty without collecting any additional aphids.
Beat "A Bridge Too Far" on extreme difficulty after fully constructing and sacrificing 14 bridges to the rising water.
In "A Bridge Too Far" on impossible difficulty, collect all food on the North-West island, and defeat the little black ant colony on the same day.
Beat the formicarium challenge "The Final Experiment".
Beat "The Final Experiment" on medium difficulty.
Beat "The Final Experiment" on hard difficulty.
Beat "The Final Experiment" on extreme difficulty.
Beat "The Final Experiment" on impossible difficulty.
Beat "The Final Experiment" without a single ant being hit by the Dragantula's charge attack.