Full list of all 46 Slay the Spire achievements. It takes around 60-80 hours to unlock all of the achievements in the base game on Windows.
The base game contains 26 achievements, and there are 8 DLC packs containing 20 achievements.
Have 999 Block during combat.
Have 50 or more Strength during combat.
Play 10 Shivs in a single turn.
Have 3 or fewer cards in hand, draw, and discard pile combined.
Defeat 3 enemies with Poison in a single combat.
Play 25 cards in a single turn.
Defeat a boss in a single turn.
Defeat a boss without taking any damage.
Have 10 or more buffs during combat.
Have 9 Energy during a single combat.
Defeated the Bronze Automaton.
Exhaust 20 cards in a single combat.
Win a battle with 1 HP remaining.
Defeated the Time Eater.
Defeated the Slime Boss.
Win a combat without playing an Attack.
Beat the game with the Silent.
Defeated Donu & Deca.
Defeated the Collector.
Apply 99 or more Poison on a single enemy.
Beat the game with the Ironclad.
Defeated the Awakened One.
Have 99 or more Block during combat.
Defeated the Champ.
Defeated Hexaghost.
Defeated the Guardian.
Obtain all other Achievements.
Complete Ascension Level 10.
Finish Donu with a Feed.
Beat the game with a 5 card deck or smaller.
Beat the game in under 20 minutes.
Beat the game with a single relic.
Unlock Ascension mode.
Have 25 or more Focus during combat.
Beat the game with a deck of only common cards.
Beat the game with the Defect.
Channel 9 Plasma in a single turn.
Win a Daily Climb.
Defeat the Transient before it fades away.
Complete Ascension Level 20.
Beat the game with the Watcher.
Complete the Ending with the Watcher.