Full list of all 46 Slay the Spire achievements. It takes around 60-80 hours to unlock all of the achievements in the base game on Windows.
The base game contains 26 achievements, and there are 8 DLC packs containing 20 achievements.
Play 10 Shivs in a single turn.
Have 999 Block during combat.
Defeat a boss in a single turn.
Play 25 cards in a single turn.
Have 3 or fewer cards in hand, draw, and discard pile combined.
Defeat a boss without taking any damage.
Have 10 or more buffs during combat.
Defeat 3 enemies with Poison in a single combat.
Have 9 Energy during a single combat.
Apply 99 or more Poison on a single enemy.
Have 50 or more Strength during combat.
Exhaust 20 cards in a single combat.
Defeated Donu & Deca.
Win a combat without playing an Attack.
Defeated the Champ.
Win a battle with 1 HP remaining.
Beat the game with the Ironclad.
Defeated the Time Eater.
Have 99 or more Block during combat.
Defeated the Collector.
Beat the game with the Silent.
Defeated the Awakened One.
Defeated the Bronze Automaton.
Defeated the Slime Boss.
Defeated Hexaghost.
Defeated the Guardian.
Obtain all other Achievements.
Complete Ascension Level 10.
Finish Donu with a Feed.
Beat the game in under 20 minutes.
Beat the game with a single relic.
Beat the game with a 5 card deck or smaller.
Unlock Ascension mode.
Beat the game with a deck of only common cards.
Have 25 or more Focus during combat.
Beat the game with the Defect.
Channel 9 Plasma in a single turn.
Win a Daily Climb.
Defeat the Transient before it fades away.
Complete Ascension Level 20.
Beat the game with the Watcher.
Complete the Ending with the Watcher.