Full list of all 46 Slay the Spire achievements. It takes around 60-80 hours to unlock all of the achievements in the base game on Windows.
The base game contains 26 achievements, and there are 8 DLC packs containing 20 achievements.
Play 10 Shivs in a single turn.
Have 50 or more Strength during combat.
Play 25 cards in a single turn.
Defeat a boss in a single turn.
Win a battle with 1 HP remaining.
Have 9 Energy during a single combat.
Win a combat without playing an Attack.
Have 10 or more buffs during combat.
Have 3 or fewer cards in hand, draw, and discard pile combined.
Have 999 Block during combat.
Have 99 or more Block during combat.
Beat the game with the Silent.
Apply 99 or more Poison on a single enemy.
Defeat 3 enemies with Poison in a single combat.
Exhaust 20 cards in a single combat.
Defeated Donu & Deca.
Defeat a boss without taking any damage.
Defeated the Bronze Automaton.
Defeated the Time Eater.
Defeated the Collector.
Defeated the Slime Boss.
Defeated Hexaghost.
Beat the game with the Ironclad.
Defeated the Awakened One.
Defeated the Champ.
Defeated the Guardian.
Obtain all other Achievements.
Beat the game in under 20 minutes.
Finish Donu with a Feed.
Beat the game with a single relic.
Complete Ascension Level 10.
Beat the game with a 5 card deck or smaller.
Unlock Ascension mode.
Have 25 or more Focus during combat.
Beat the game with a deck of only common cards.
Beat the game with the Defect.
Channel 9 Plasma in a single turn.
Win a Daily Climb.
Defeat the Transient before it fades away.
Complete Ascension Level 20.
Beat the game with the Watcher.
Complete the Ending with the Watcher.