Full list of all 46 Slay the Spire achievements. It takes around 60-80 hours to unlock all of the achievements in the base game on Windows.
The base game contains 26 achievements, and there are 8 DLC packs containing 20 achievements.
Have 3 or fewer cards in hand, draw, and discard pile combined.
Defeat 3 enemies with Poison in a single combat.
Play 10 Shivs in a single turn.
Have 999 Block during combat.
Play 25 cards in a single turn.
Apply 99 or more Poison on a single enemy.
Defeat a boss in a single turn.
Win a combat without playing an Attack.
Have 99 or more Block during combat.
Defeated the Time Eater.
Have 10 or more buffs during combat.
Beat the game with the Silent.
Defeated Donu & Deca.
Have 50 or more Strength during combat.
Defeated the Collector.
Win a battle with 1 HP remaining.
Exhaust 20 cards in a single combat.
Have 9 Energy during a single combat.
Defeated the Bronze Automaton.
Defeat a boss without taking any damage.
Defeated the Slime Boss.
Defeated Hexaghost.
Beat the game with the Ironclad.
Defeated the Awakened One.
Defeated the Champ.
Defeated the Guardian.
Obtain all other Achievements.
Beat the game with a 5 card deck or smaller.
Beat the game in under 20 minutes.
Beat the game with a single relic.
Finish Donu with a Feed.
Complete Ascension Level 10.
Unlock Ascension mode.
Have 25 or more Focus during combat.
Beat the game with a deck of only common cards.
Beat the game with the Defect.
Channel 9 Plasma in a single turn.
Win a Daily Climb.
Defeat the Transient before it fades away.
Complete Ascension Level 20.
Beat the game with the Watcher.
Complete the Ending with the Watcher.