Full list of all 46 Slay the Spire achievements. It takes around 60-80 hours to unlock all of the achievements in the base game on Windows.
The base game contains 26 achievements, and there are 8 DLC packs containing 20 achievements.
Have 999 Block during combat.
Have 99 or more Block during combat.
Apply 99 or more Poison on a single enemy.
Play 25 cards in a single turn.
Have 10 or more buffs during combat.
Defeat a boss in a single turn.
Defeat a boss without taking any damage.
Defeated Donu & Deca.
Win a combat without playing an Attack.
Defeated the Time Eater.
Have 50 or more Strength during combat.
Beat the game with the Ironclad.
Defeated the Bronze Automaton.
Beat the game with the Silent.
Defeated the Awakened One.
Play 10 Shivs in a single turn.
Exhaust 20 cards in a single combat.
Defeated the Champ.
Have 9 Energy during a single combat.
Defeated the Collector.
Defeat 3 enemies with Poison in a single combat.
Win a battle with 1 HP remaining.
Defeated the Slime Boss.
Defeated Hexaghost.
Defeated the Guardian.
Have 3 or fewer cards in hand, draw, and discard pile combined.
Obtain all other Achievements.
Beat the game with a single relic.
Beat the game with a 5 card deck or smaller.
Complete Ascension Level 10.
Finish Donu with a Feed.
Beat the game in under 20 minutes.
Unlock Ascension mode.
Have 25 or more Focus during combat.
Beat the game with a deck of only common cards.
Beat the game with the Defect.
Channel 9 Plasma in a single turn.
Win a Daily Climb.
Defeat the Transient before it fades away.
Complete Ascension Level 20.
Beat the game with the Watcher.
Complete the Ending with the Watcher.