Full list of all 54 Enter the Gungeon achievements. It takes around 120-150 hours to unlock all of the achievements in the base game on Windows.
The base game contains 48 achievements, and there are 2 DLC packs containing 6 achievements.
Complete Frifle's Challenges
Kill 100 enemies while riding in a mine cart
Steal 10 things
Kill 100 enemies by dropping chandeliers
Complete the game with the Sorceress's Enchanted Gun
Collect five Master Rounds in one run
Ace Winchester's game 3 times
Clear the Sixth Chamber
Open the shortcut to the Fifth Chamber
You've met with a terrible fate, haven't you
Kill a Jammed Boss
Complete the game with Beast Mode on
Complete all 4 main character Pasts
Kill the Pilot's Past
Kill the Convict's Past
Unlock the Robot
Carry a large amount of money at once
Accessed the Hidden Market
Open the shortcut to the Fourth Chamber
Clear the Fifth Chamber 10 Times
Clear the Fourth Chamber 20 Times
Populate the Breach
Avenge Manuel
Kill the Hunter's Past
Clear the Third Chamber 30 Times
Clear the Second Chamber 40 Times
Why
Unlock the Bullet
Die in the Past
Fall at the last second
Open the shortcut to the Third Chamber
Win a wager against the Gunsling King 5 times
Deliver the Golem's replacement arm
Kill the Marine's Past
Map the first Five Chambers for the lost adventurer
Construct the Bullet that can kill the Past
Always be flipping. Guns are for flippers
Kill 100 enemies by knocking them into pits
Clear the First Chamber 50 Times
Kill a boss after covering it with glitter
Spend big at the Acquisitions Department
Defeat the Boss of the Fifth Chamber
Get the jump on a Mimic
Access the Temple
Open the shortcut to the Second Chamber
Kill a frozen enemy by rolling into it
Access the Oubliette
Complete the Tutorial
Kill the Robot's Past
Kill the Bullet's Past
Complete Daisuke's trial
Defeat an Advanced Boss
Take Revenge
Complete Tonic's Challenge